WebBiotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; … WebCTP is an essential nucleotide that is required for the biosynthesis of nucleic acids[1]and protein glycosylation[2].In addition,it also serves as a critical precursor during the synthesis of membrane phospholipids[3]and participates in cellular signal communication[4].While ATP serves as the phosphate donor for most kinases,numerous studies ...

Biotin-11-CTP PerkinElmer

WebBiotin-11-CTP (Biotin-11-cytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into RNA 10 mM solution in pH 7.5 Tris-HCl buffer Store at -20°C WebBiotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP. The biotinylated RNA can be used as a probe in membrane-based or in situhybridizations. The amount of material provided is sufficient for up to 20 transcription ... resthof celle

CAS：17796-82-6 N-环己基硫代酞酰亚胺-德尔塔(Delta)生物试剂

WebAfter incorporating a pulse of a labelled nucleotide , such as 5-bromouridine 5′-triphosphate (BrUTP) or biotin-CTP, into permeabilized mammalian cells, Cook and colleaguesnoted … WebJul 21, 2016 · 2-Biotin run-on 2× reaction mix. i. Transfer a 28-μl aliquot of NRO master mix to a microcentrifuge tube. ii. Add 5 μl each of biotin-11-CTP (1 mM) and biotin-11-UTP (1 mM) to the NRO master ... Web作为基于FRET的ATP荧光探针，FoF 1-ATP合成酶的ε亚基具有几个突出优点：ε亚基与ATP结合，但不水解ATP；结合ATP，但不结合ADP、GTP、CTP以及UTP；当ATP结合时，ε亚基发生很大的构象变化形成一个折叠的构象(folded form)，从而使N端的青色荧光蛋白以及C端的黄色荧光蛋白 ... resthof bremen