WebS6651S 20 ml: Catalog # Size; S6651L 100 ml: S6651S ... customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further ... Stored at (°C) Amount: Concentration: S6651S: 4 : Chitin Resin: S6651SVIAL: 4: 1 x 20 ml: S6651L: 4 : Chitin Resin: S6651LVIAL: 4: 1 x 100 ml ... WebA chitin affinity matrix is used to isolate the fusion precursor that contains the target protein, intein and a chitin bindin g domain (CBD). 20 ml of Chitin Resin (NEB #S6651) are supplied as a 40 ml slurry in 20% ethanol. The binding capacity for the intein tag fused to the target protein, maltose binding protein (MBP), is 2 mg of eluted MBP ...
IMPACT Kit E6901 manual - NEB
WebProduct Name: Chitin Resin Catalog #: S6651S/L Shelf Life: 36 months Storage Temp: 4°C Specification Version: PS-S6651S/L v1.0 Effective Date: 15 Jun 2024 Assay Name/Specification (minimum release criteria) Functional Binding Assay (Resin Binding Capacity) - Chitin Resin ( 1 ml ) was packed into a column and equilibrated with column … WebApr 29, 2024 · A 2.5 mL aliquot of chitin slurry resin (NEB, S6651S) was packed into each of two disposable columns (Bio-rad 7321010). Columns were washed with 20 mL of HEGX Buffer. The soluble fraction was added to the chitin resin slowly, then incubated on a rotator at 4 °C overnight. イエウール 評判
Protocol for Removal of IMAC Contaminating Proteins (C2529) - NEB
WebNov 16, 2024 · Preparation of con A-coated beads. Four different streptavidin-conjugated Dynabeads, M-270, M-280, MyOne C1, and MyOne T1 that are capable of binding to biotin-conjugated concanavalin A (con A) were purchased from Thermo Fisher ().To conjugate con A, 100 μL of each beads is washed with 1× PBS (pH 6.8) for three times and … WebS6651S. 20 ml. $184.00. $165.60. *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca. An affinity matrix for the … Webpassed over a chitin column. The protein of interest elutes in the flow through (FT), while the CBD-tagged metal binding proteins remain bound (B) to the chitin resin (NEB #S6651S). Purifications were performed according to manufacturers' recommended conditions. B) Contaminants ArnA, SlyD and Can are confirmed イエウール 東京