WebJan 6, 2016 · In an enhanced GFP disruption assay, this Cas9 mutant showed greater than 90 percent efficiency. The scientists named this protein SpCas9 high-fidelity variant number 1 (SpCas9-HF1) and went on to show that it had comparable on-target efficiency compared to wild-type SpCas9 when used to target both EGFP and several endogenous … WebAssay Principle Principle of the plug and play GFP Transfection Efficiency Assay using the NucleoCounter® NC-3000™: Cells are located using Hoechst 33342 (blue) and the percentage of GFP expressing cells (green) can easily be determined. Non-viable cells are stained with propidium iodide (PI; red)
High-performance CRISPR-Cas12a genome editing for ... - Nature
WebMar 3, 2024 · A GFP disruption assay was used by Fu et al. 15 and demonstrated inconsistent Cas9 activity at three different sites of the EGFP gene. Multiple gRNAs for each site were designed, each with a mismatch in a different position, in order to prepare a specificity profile for SpCas9. WebJan 21, 2024 · Fluorescent protein disruption assay Fluorescent protein disruption assays were performed to evaluate DNA cleavage and indel-mediated disruption at the target site of the fluorescent protein (i.e. GFP) brought by SaCas9 and gRNA expressions, which results in loss of cell fluorescence. atkins penne pasta
High-throughput and high-content bioassay enables tuning of …
WebForced expression of SOCS1 attenuates LPS-induced endothelial barrier disruption and inflammation. A ... EB1 or β-tubulin and Myc-SOCS1 were tested in co-IP assay with GFP antibody followed by immunoblotting with Myc-antibody. Probing of total cell lysates with GFP and Myc antibodies was used to verify ectopic protein expression; membrane re ... WebBenzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon, is considered a common endocrine disrupting chemical (EDC) with mutagenic and carcinogenic effects. In this work, we evaluated the effects of BaP on the hypothalamo-pituitary-gonadal axis (HPG) of zebrafish embryos. The embryos were treated with 5 and 50 nM BaP from 2.5 to 72 … WebAssays, Gene delivery, Genetics, Imaging probes, Polyethylenimine Abstract Carbon dots (CDs) have become the focus of many studies due to their outstanding optical properties and good biocompatibility. We investigated their potential application to produce a smart and highly efficient yet nontoxic nanovector for gene delivery. atkins penne pasta nutrition