Ptrc-hisa
WebPlasmid pTrcHisA-PPARalpha(aa101-468) from Dr. Martin Scanlon's lab contains the insert human peroxisome proliferator activated receptor alpha A/B domain deletion construct … WebAn object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in …
Ptrc-hisa
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WebThe wells containing bilayers were washed with RPMI culture media and warmed to 37°C for 30 min prior to adding the cells. Cells were added to the bilayer at a density of 250,000 cells/well for 4–5 min at 37°C and fixed using 4% PFA in PBS warmed to 37°C. Fixation of cells was done for 15 min at 37°C.
WebWe constructed an expression plasmid, using the vector (pTrc-HisA) and the monkey cDNA for the 290-amino-acid polypeptide. The recombinant protein with a M(r) of 33 kDa exhibited PGE synthase activity and was purified to apparent homogeneity by nickel-chelating column chromatography. The V(max) and K(m) values for PGH(2) of the purified ... WebVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that …
WebNational Center for Biotechnology Information WebpTrcHisA-SC TeTx WT or E234A were used to inoculate 10 ml of Luria-Bertani (LB) medium, and grown overnight with agitation (200 rpm) at 37 °C. An aliquot (2 ml) of each culture was transferred to 200 ml of fresh LB medium with the addition of ampicillin (100 g/ml); the cells were grown further at 37 °C with agitation to reach A 600 nm 0.5 ...
WebpTrcHisA载体简介. pTrcHis载体是来由pBR322质粒衍生而来的表达载体,设计用来在大肠杆菌中进行重组蛋白的高效表达和纯化。. 载体上的Trc启动子和rrnB抗终止区使得高水平蛋白表达成为可能.Trc启动子由Trp启动子的-35区域和Lac启动子的-10区域共同组成。. pTrcHis载体同 …
WebMar 27, 2024 · Texas Railroad Commission - 03-27-2024. 2024-0171-oxy-waiver-zaplock-phmsas-response-txrrc-final-amendment-005.pdf (193.66 KB) DOT is committed to … hops with pineapple notesWebOur pTrcHis A, B, & C vectors are designed to offer enhanced translation initiation and high-level expression in E. coli. These vectors feature: • High-level regulated transcription from … lookism chapter 341Webfragment from pUC-IIC2 was transferred to pTrc-HisA that had been digested with Nco I and Eco RI, for expression of IIC2 (residues 821 to 1090 of type 11 adenylyl cyclase). To link IC1 and IIC2, we ligated the 0.9-kb Bsp HI-Eco RI fragment from pUC-IIC2 with phosphorylated linkers (5'-GGCCGCTGGAGG and 5'-GATGCCTCCAGC) and pTrc-IsC1 that had been lookism chapter 372WebNov 7, 2012 · His-tagged constructs for Cbl-c, Cbl-c Y341E, Hic-5, Hic-5 C287A, Hic-5 LIM2, and Hic-5 LIM2 C287A were created by amplifying the appropriate sequences using PCR and cloning the PCR products into the pTrc-HisA vector at the XhoI and HindIII restriction sites. pTrc-HisA was purchased from Invitrogen (Carlsbad, CA). pET15b-UbcH5b was a … lookism chapter 367WebMar 31, 2011 · Severe congenital neutropenia (SCN) is an inborn disorder of granulopoiesis that in many cases is caused by mutations of the ELANE gene, which encodes neutrophil elastase (NE). Recent data suggest a model in which ELANE mutations result in NE protein misfolding, induction of endoplasmic reticulum (ER) stress, activation of the unfolded … hops wow tbcWebpTrcHisA vector; pTrcHisA vector. Cat.No. VET1033. Promoter. Trc. Selection. Ampicillin. Source. Escherichia coli. Species. Bacterial. Tag. N-His, N-EK. Q&A Customer Reviews. … lookism chapter 395 englishWebMar 8, 2002 · We constructed an expression plasmid, using the vector (pTrc-HisA) and the monkey cDNA for the 290-amino-acid polypeptide. The recombinant protein with a M r of 33 kDa exhibited PGE synthase activity and was purified to apparent homogeneity by nickel-chelating column chromatography. lookism chapter 378