Smmc 7221
Web22 Feb 2024 · Two micrograms of total RNA extracted from LO2, HepG2, and SMMC-7221 cells were reverse-transcribed using a GoScript reverse transcription kit (Promega) follow the manufacturer’s instructions. Quantitative RT-PCR was performed following procedures described previously and the expression levels of each gene were normalized to GADPH … WebConclusions: Xylitol/selenite is a promising anti-cancer drug to induce apoptosis in SMMC-7221 cells. It may regulate the apoptosis through the co-action of multiple mechanisms related to GSH depletion. Keywords: Anti-cancer; Apoptosis; Capillary electrophoresis; Glutathione; Laser-induce fluorescence; Selenium; Xylitol/selenite. Publication types
Smmc 7221
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WebThe SMMC-7221 and Huh7 cells (2 × 10 6 cells/ml) were seeded into 24-well plates with 2-well Ibidi culture-inserts (Ibidi, Munich, Germany). After incubation for 24 h, the culture-inserts were removed carefully. The wounds were observed and their widths were determined using an inverted microscope. WebD Morphological changes of the four groups of SMMC-7221 and HepG2 cells. E The expression levels of EMT markers were detected by Western blotting in SMMC-7221 and HepG2 cells with NQO1...
Web1 Aug 2024 · It is considered as a promising therapeutic target against cancer. (TCM) and its bioactive components have been used in cancer therapy for an extended period, and they are well-known for their multi-target pharmacological functions and fewer side effects. WebCompounds 6, 18 and 22 exhibited interesting activities towards all cell line tested, while Compounds 1, 4, 15 and 22 showed strong activity towards SMMC-7221 cell line in the range of 1–2 μg/mL IC 50. Additionally, we have further studied three Compounds 6, 18 and 22 as representative of all these analogues to understand the pathway.
Web2 Mar 2024 · Moreover, treatment with 5–80 μM rhein may decrease the energy metabolism of SMMC-7221 and SMMC-7221/doxorubicin-resistant cells by inhibiting oxidative phosphorylation. However, CsA cannot reverse the inhibition of energy metabolism via rhein (Wu et al., 2024). Web27 Jun 2012 · SMMC-7721, a human hepatoma carcinoma (HCC) cell line, was cultured in Dulbecco’s modified Eagle medium (DMEM, Invitrogen Corp., Grand Island, New York) supplemented with 10% FBS at 37°C under a mixture of 95% air and 5% CO 2. ... (separate comparisons for PC-3 and SMMC-7221 mouse models). Similarly, one-way ANOVA was …
Web31 Dec 2010 · INTRODUCTION. Lysophosphatidic acid (LPA), also known as an important lipid mediator, is a potent regulator of proliferation and migration in many cell lines (Van Meeteren and Moolenaar, 2007; Pua et al., 2009; Liu et al., 2010; Wang et al., 2010).Extracellular LPA is thought to be produced primarily by the autotaxin …
Web20 Jun 2016 · A series of pyrazolo[3,4-d]pyrimidine derivatives related to allopurinol has been synthesized and evaluated for its cytotoxicity against a panel of three cancer cell lines as well as its xanthine oxidase (XOD) inhibitory activities. Among them, compound 4 showed potent cytotoxicity with IC50 values of 25.5 and 35.2 μM against human hepatoma … sakura gelly roll complete pen setWeb1 Jan 2024 · SMMC-7221 and HepG2 cells were transiently transfected with miR-542-3p mimics, miR-542-3p inhibitor, or miR-NC. MTT assay of SMMC-7221 (C) and HepG2 (D) cells at 24, 48, and 72 h of transfection. * p < 0.5. (E) The colony-forming capacity of HCC cells was detected by colony formation assay after incubation for 2 weeks. * p < 0.5 vs. miR-NC. things rtWebIn present study, we found that monocyte cell line THP-1 cells were more sensitive to Icaritin than tumor cell lines such as SMMC-7221 (Fig. 4C). We speculate that Icaritin may exert its effect mainly on host immune cells, particularly those with monocyte lineage, such as macrophages and myeloid DCs, which activate T cells by antigen presenting. sakura gelly roll moonlight 06Web16 Oct 2024 · They were subcutaneously inoculated with SMMC-7221 cells (5 * 10 6) stably expressing control lentivirus and shPYCR1 lentivirus, respectively. After the diameter of tumor was larger than 5 mm, tumor volume was determined twice per week. The tumor volume was calculated as V = (width 2 * length)/2. The bioluminescence imaging was … sakura gelly roll color chartWeb5 Feb 2024 · The apoptotic rates of HepG2 and SMMC-7221 cells treated with brevilin A were found to be markedly increased. The expression levels of MMP-2 and MMP-9 were … sakura gelly roll pens hobby lobbyWeb20 Nov 2024 · The expression of TUFT1 in SMMC-7221 cell lines was down-regulated by lentiviral vector. Cell proliferation assay, clonogenic assay, cell apoptosis assay and cell … sakura gelly roll pens clearanceWebA, TPM4 levels in HCC tissues and paracancerous tissues. B, TPM4 levels in normal hepatocytes L02 and liver ship between the expression levels of TPM4 and cancer cells (Hep3B, Huh7, SMMC-7221, MHCC88H, HepG2 SUSD2 in HCC tissues. p. 0.05 was considered and Bel-7402). C, Survival of HCC patients expressing high or statistically … sakura gelly roll white hobby lobby